The program Target_miRNA is intended for prediction of microRNA targets in user RNA sequence. MicroRNA (miRNA) is a form of single-stranded RNA which is typically 20-25 nucleotides long, and is thought to regulate the expression of other genes. The target is an area in RNA sequence, which is appropriate for microRNA to form a stable secondary structure with it. Target_miRNA program scans thes equence and calculates microRNA-Target complex secondary structure and energy. If energy is lower than threshold than this position goes to the output.