TBLASTX is a powerful gene-prediction tool for genomes that are appropriately diverged.
TBLASTX translates both strands of the query and nucleotide database sequences in three frames on each strand, and examine all pairwise combinations to find similarities at the amino acid level.
Net-tBlastX is a variant of the tBlastX program intended for work with distant databases.
!NOTE! Because this program involves more computation than the others, it is not recommended to search of the Non-redundant (nr) database.
BLAST is a service of the National Center for Biotechnology Information (NCBI). A nucleotide or protein sequence sent to the BLAST server is compared against databases at the NCBI and a summary of matches is returned to the user.
The www BLAST server can be accessed through the home page of the NCBI at Stand-alone BLAST binaries can be obtained from the NCBI FTP site.

Net-tBlastX description

Net-tBlastX parameters