|
|
|
|
Fgenesh |
Program for predicting multiple genes in genomic DNA sequences.
Fgenesh is the fastest (50-100 times faster than GenScan) and most accurate gene finder available (see: Figure and Table, respectively). In recent rice genome sequencing projects, it was cited "the most successful (gene finding) program (Yu et al. (2002) Science 296:79) and was used to produce 87% of all high-evidence predicted genes (Goff et al. (2002) Science 296:79).
Figure. Performance of different gene finding programs on rice genes (reprinted from Yu et al., 2002, Science, 296:79-92). These tests confirmed that Fgenesh is by far the most accurate program (of five programs tested).
Table. Performance of three popular gene prediction programs on 42 semi-artificial genomic sequences containing 178 known human gene sequences (900 exons). Sensitivity is percentage of exons that are predicted correctly. Selectivity is percentage of predicted exons that are correct (these results reproduced with some changes from Yada et al., 2002, Cold Spring Harbor Genome Sequencing and Biology Meeting, May 7-11). These tests demonstrated that Fgenesh is by far the most accurate program (of three programs tested).
| Program |
Sensitivity
|
Specificity
|
Missed Exons, %
|
Wrong Exons, %
|
| Fgenesh |
77.1
|
65.7
|
9.6
|
23.2
|
| GenScan |
66.5
|
44.9
|
12.0
|
40.9
|
| HMMGene |
69.6
|
36.6
|
15.5
|
55.5
|
References: Salamov A., Solovyev V. (2000)
Ab initio gene finding in Drosophila genomic DNA.
Genome Res., 10,516-522
Fgenesh output:
FGENESH 2.6 Prediction of potential genes in Homo_sapiens genomic DNA Time : Thu Dec 27 19:47:24 2007 Seq name: gi|13907843|ref|NG_000007.1| Homo sapiens genomic beta globin region (HBB@) on chromosome 11 Length of sequence: 73308 Number of predicted genes 10: in +chain 10, in -chain 0. Number of predicted exons 21: in +chain 21, in -chain 0. Positions of predicted genes and exons: Variant 1 from 1, Score:180.171899 G Str Feature Start End Score ORF Len 1 + TSS 19456 -7.09 1 + 1 CDSf 19541 - 19632 16.13 19541 - 19630 90 1 + 2 CDSi 19755 - 19977 13.37 19756 - 19977 222 1 + 3 CDSl 20833 - 20961 3.34 20833 - 20961 129 1 + PolA 21055 1.13 2 + TSS 34446 -7.09 2 + 1 CDSf 34531 - 34622 13.42 34531 - 34620 90 2 + 2 CDSi 34745 - 34967 21.52 34746 - 34967 222 2 + 3 CDSl 35854 - 35982 2.92 35854 - 35982 129 2 + PolA 36043 1.13 3 + TSS 39382 -7.09 3 + 1 CDSf 39467 - 39558 13.42 39467 - 39556 90 3 + 2 CDSi 39681 - 39903 21.52 39682 - 39903 222 3 + 3 CDSl 40770 - 40898 3.66 40770 - 40898 129 3 + PolA 40959 1.13 4 + TSS 44415 -8.69 4 + 1 CDSf 45995 - 46151 16.58 45995 - 46150 156 4 + 2 CDSl 46997 - 47100 -1.94 46999 - 47100 102 4 + PolA 47243 1.13 5 + TSS 54707 -4.39 5 + 1 CDSf 54790 - 54881 13.44 54790 - 54879 90 5 + 2 CDSi 55010 - 55232 17.01 55011 - 55232 222 5 + 3 CDSl 56425 - 56535 2.53 56425 - 56535 111 5 + PolA 56931 1.13 6 + TSS 62104 -6.59 6 + 1 CDSf 62187 - 62278 12.99 62187 - 62276 90 6 + 2 CDSi 62409 - 62631 20.06 62410 - 62631 222 6 + 3 CDSl 63482 - 63610 9.54 63482 - 63610 129 6 + PolA 63718 1.13 7 + TSS 68088 -9.39 7 + 1 CDSo 68183 - 68428 19.52 68183 - 68428 246 7 + PolA 68509 1.13 8 + TSS 69336 -10.29 8 + 1 CDSo 69467 - 70072 16.45 69467 - 70072 606 8 + PolA 70131 -1.08 9 + TSS 70224 -12.49 9 + 1 CDSo 70355 - 70819 17.10 70355 - 70819 465 9 + PolA 70905 1.13 10 + TSS 72085 -6.39 10 + 1 CDSo 72135 - 72395 7.31 72135 - 72395 261 10 + PolA 72952 1.13 Predicted protein(s): >FGENESH:[mRNA] 1 3 exon (s) 19541 - 20961 444 bp, chain + ATGGTGCATTTTACTGCTGAGGAGAAGGCTGCCGTCACTAGCCTGTGGAGCAAGATGAAT GTGGAAGAGGCTGGAGGTGAAGCCTTGGGCAGACTCCTCGTTGTTTACCCCTGGACCCAG AGATTTTTTGACAGCTTTGGAAACCTGTCGTCTCCCTCTGCCATCCTGGGCAACCCCAAG GTCAAGGCCCATGGCAAGAAGGTGCTGACTTCCTTTGGAGATGCTATTAAAAACATGGAC AACCTCAAGCCCGCCTTTGCTAAGCTGAGTGAGCTGCACTGTGACAAGCTGCATGTGGAT CCTGAGAACTTCAAGCTCCTGGGTAACGTGATGGTGATTATTCTGGCTACTCACTTTGGC AAGGAGTTCACCCCTGAAGTGCAGGCTGCCTGGCAGAAGCTGGTGTCTGCTGTCGCCATT GCCCTGGCCCATAAGTACCACTGA >FGENESH:[exon] Gene: 1 Exon: 1 Pos: 19541 - 19632 92 bp., chain + ATGGTGCATTTTACTGCTGAGGAGAAGGCTGCCGTCACTAGCCTGTGGAGCAAGATGAAT GTGGAAGAGGCTGGAGGTGAAGCCTTGGGCAG >FGENESH:[exon] Gene: 1 Exon: 2 Pos: 19755 - 19977 223 bp., chain + ACTCCTCGTTGTTTACCCCTGGACCCAGAGATTTTTTGACAGCTTTGGAAACCTGTCGTC TCCCTCTGCCATCCTGGGCAACCCCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGACTTC CTTTGGAGATGCTATTAAAAACATGGACAACCTCAAGCCCGCCTTTGCTAAGCTGAGTGA GCTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAG >FGENESH:[exon] Gene: 1 Exon: 3 Pos: 20833 - 20961 129 bp., chain + CTCCTGGGTAACGTGATGGTGATTATTCTGGCTACTCACTTTGGCAAGGAGTTCACCCCT GAAGTGCAGGCTGCCTGGCAGAAGCTGGTGTCTGCTGTCGCCATTGCCCTGGCCCATAAG TACCACTGA >FGENESH: 1 3 exon (s) 19541 - 20961 147 aa, chain + MVHFTAEEKAAVTSLWSKMNVEEAGGEALGRLLVVYPWTQRFFDSFGNLSSPSAILGNPK VKAHGKKVLTSFGDAIKNMDNLKPAFAKLSELHCDKLHVDPENFKLLGNVMVIILATHFG KEFTPEVQAAWQKLVSAVAIALAHKYH >FGENESH:[mRNA] 2 3 exon (s) 34531 - 35982 444 bp, chain + ATGGGTCATTTCACAGAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAAT GTGGAAGATGCTGGAGGAGAAACCCTGGGAAGGCTCCTGGTTGTCTACCCATGGACCCAG AGGTTCTTTGACAGCTTTGGCAACCTGTCCTCTGCCTCTGCCATCATGGGCAACCCCAAA GTCAAGGCACATGGCAAGAAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGAT GATCTCAAGGGCACCTTTGCCCAGCTGAGTGAACTGCACTGTGACAAGCTGCATGTGGAT CCTGAGAACTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCGGC AAAGAATTCACCCCTGAGGTGCAGGCTTCCTGGCAGAAGATGGTGACTGGAGTGGCCAGT GCCCTGTCCTCCAGATACCACTGA >FGENESH:[exon] Gene: 2 Exon: 1 Pos: 34531 - 34622 92 bp., chain + ATGGGTCATTTCACAGAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAAT GTGGAAGATGCTGGAGGAGAAACCCTGGGAAG >FGENESH:[exon] Gene: 2 Exon: 2 Pos: 34745 - 34967 223 bp., chain + GCTCCTGGTTGTCTACCCATGGACCCAGAGGTTCTTTGACAGCTTTGGCAACCTGTCCTC TGCCTCTGCCATCATGGGCAACCCCAAAGTCAAGGCACATGGCAAGAAGGTGCTGACTTC CTTGGGAGATGCCATAAAGCACCTGGATGATCTCAAGGGCACCTTTGCCCAGCTGAGTGA ACTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAG >FGENESH:[exon] Gene: 2 Exon: 3 Pos: 35854 - 35982 129 bp., chain + CTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCGGCAAAGAATTCACCCCT GAGGTGCAGGCTTCCTGGCAGAAGATGGTGACTGGAGTGGCCAGTGCCCTGTCCTCCAGA TACCACTGA >FGENESH: 2 3 exon (s) 34531 - 35982 147 aa, chain + MGHFTEEDKATITSLWGKVNVEDAGGETLGRLLVVYPWTQRFFDSFGNLSSASAIMGNPK VKAHGKKVLTSLGDAIKHLDDLKGTFAQLSELHCDKLHVDPENFKLLGNVLVTVLAIHFG KEFTPEVQASWQKMVTGVASALSSRYH >FGENESH:[mRNA] 3 3 exon (s) 39467 - 40898 444 bp, chain + ATGGGTCATTTCACAGAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAAT GTGGAAGATGCTGGAGGAGAAACCCTGGGAAGGCTCCTGGTTGTCTACCCATGGACCCAG AGGTTCTTTGACAGCTTTGGCAACCTGTCCTCTGCCTCTGCCATCATGGGCAACCCCAAA GTCAAGGCACATGGCAAGAAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGAT GATCTCAAGGGCACCTTTGCCCAGCTGAGTGAACTGCACTGTGACAAGCTGCATGTGGAT CCTGAGAACTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCGGC AAAGAATTCACCCCTGAGGTGCAGGCTTCCTGGCAGAAGATGGTGACTGCAGTGGCCAGT GCCCTGTCCTCCAGATACCACTGA >FGENESH:[exon] Gene: 3 Exon: 1 Pos: 39467 - 39558 92 bp., chain + ATGGGTCATTTCACAGAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAAT GTGGAAGATGCTGGAGGAGAAACCCTGGGAAG >FGENESH:[exon] Gene: 3 Exon: 2 Pos: 39681 - 39903 223 bp., chain + GCTCCTGGTTGTCTACCCATGGACCCAGAGGTTCTTTGACAGCTTTGGCAACCTGTCCTC TGCCTCTGCCATCATGGGCAACCCCAAAGTCAAGGCACATGGCAAGAAGGTGCTGACTTC CTTGGGAGATGCCATAAAGCACCTGGATGATCTCAAGGGCACCTTTGCCCAGCTGAGTGA ACTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAG >FGENESH:[exon] Gene: 3 Exon: 3 Pos: 40770 - 40898 129 bp., chain + CTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCGGCAAAGAATTCACCCCT GAGGTGCAGGCTTCCTGGCAGAAGATGGTGACTGCAGTGGCCAGTGCCCTGTCCTCCAGA TACCACTGA >FGENESH: 3 3 exon (s) 39467 - 40898 147 aa, chain + MGHFTEEDKATITSLWGKVNVEDAGGETLGRLLVVYPWTQRFFDSFGNLSSASAIMGNPK VKAHGKKVLTSLGDAIKHLDDLKGTFAQLSELHCDKLHVDPENFKLLGNVLVTVLAIHFG KEFTPEVQASWQKMVTAVASALSSRYH >FGENESH:[mRNA] 4 2 exon (s) 45995 - 47100 261 bp, chain + ATGGGCAACCCCAAAGTCAAGGCACATGGCAAGAAGGTGCTGATCTCCTTCGGAAAAGCT GTTATGCTCACGGATGACCTCAAAGGCACCTTTGCTACACTGAGTGACCTGCACTGTAAC AAGCTGCACGTGGACCCTGAGAACTTCCTGGTGAGTACTCTTAGGCAACGTGATATTGAT TGTTTTGGCAACCCACTTCAGCGAGGATTTTACCCTACAGATACAGGCTTCTTGGCAGTA ACTAACAAATGCTGTGGTTAA >FGENESH:[exon] Gene: 4 Exon: 1 Pos: 45995 - 46151 157 bp., chain + ATGGGCAACCCCAAAGTCAAGGCACATGGCAAGAAGGTGCTGATCTCCTTCGGAAAAGCT GTTATGCTCACGGATGACCTCAAAGGCACCTTTGCTACACTGAGTGACCTGCACTGTAAC AAGCTGCACGTGGACCCTGAGAACTTCCTGGTGAGTA >FGENESH:[exon] Gene: 4 Exon: 2 Pos: 46997 - 47100 104 bp., chain + CTCTTAGGCAACGTGATATTGATTGTTTTGGCAACCCACTTCAGCGAGGATTTTACCCTA CAGATACAGGCTTCTTGGCAGTAACTAACAAATGCTGTGGTTAA >FGENESH: 4 2 exon (s) 45995 - 47100 86 aa, chain + MGNPKVKAHGKKVLISFGKAVMLTDDLKGTFATLSDLHCNKLHVDPENFLVSTLRQRDID CFGNPLQRGFYPTDTGFLAVTNKCCG >FGENESH:[mRNA] 5 3 exon (s) 54790 - 56535 426 bp, chain + ATGGTGCATCTGACTCCTGAGGAGAAGACTGCTGTCAATGCCCTGTGGGGCAAAGTGAAC GTGGATGCAGTTGGTGGTGAGGCCCTGGGCAGATTACTGGTGGTCTACCCTTGGACCCAG AGGTTCTTTGAGTCCTTTGGGGATCTGTCCTCTCCTGATGCTGTTATGGGCAACCCTAAG GTGAAGGCTCATGGCAAGAAGGTGCTAGGTGCCTTTAGTGATGGCCTGGCTCACCTGGAC AACCTCAAGGGCACTTTTTCTCAGCTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGAT CCTGAGAACTTCAGGGTGTGTAAGAAGGTTCCTGAGGCTCTACAGATAGGGAGCACTTGT TTATTTTACAAAGAGTACATGGGAAAAGAGAAAAGCAAGGGAACCGTACAAGGCATTAAT GGGTGA >FGENESH:[exon] Gene: 5 Exon: 1 Pos: 54790 - 54881 92 bp., chain + ATGGTGCATCTGACTCCTGAGGAGAAGACTGCTGTCAATGCCCTGTGGGGCAAAGTGAAC GTGGATGCAGTTGGTGGTGAGGCCCTGGGCAG >FGENESH:[exon] Gene: 5 Exon: 2 Pos: 55010 - 55232 223 bp., chain + ATTACTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCTC TCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAGGTGCTAGGTGC CTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACTTTTTCTCAGCTGAGTGA GCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGG >FGENESH:[exon] Gene: 5 Exon: 3 Pos: 56425 - 56535 111 bp., chain + GTGTGTAAGAAGGTTCCTGAGGCTCTACAGATAGGGAGCACTTGTTTATTTTACAAAGAG TACATGGGAAAAGAGAAAAGCAAGGGAACCGTACAAGGCATTAATGGGTGA >FGENESH: 5 3 exon (s) 54790 - 56535 141 aa, chain + MVHLTPEEKTAVNALWGKVNVDAVGGEALGRLLVVYPWTQRFFESFGDLSSPDAVMGNPK VKAHGKKVLGAFSDGLAHLDNLKGTFSQLSELHCDKLHVDPENFRVCKKVPEALQIGSTC LFYKEYMGKEKSKGTVQGING >FGENESH:[mRNA] 6 3 exon (s) 62187 - 63610 444 bp, chain + ATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAAC GTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTACCCTTGGACCCAG AGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATGCTGTTATGGGCAACCCTAAG GTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGAC AACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGAT CCTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGC AAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAAT GCCCTGGCCCACAAGTATCACTAA >FGENESH:[exon] Gene: 6 Exon: 1 Pos: 62187 - 62278 92 bp., chain + ATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAAC GTGGATGAAGTTGGTGGTGAGGCCCTGGGCAG >FGENESH:[exon] Gene: 6 Exon: 2 Pos: 62409 - 62631 223 bp., chain + GCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCAC TCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC CTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGA GCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGG >FGENESH:[exon] Gene: 6 Exon: 3 Pos: 63482 - 63610 129 bp., chain + CTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCA CCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAG TATCACTAA >FGENESH: 6 3 exon (s) 62187 - 63610 147 aa, chain + MVHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPK VKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFG KEFTPPVQAAYQKVVAGVANALAHKYH >FGENESH:[mRNA] 7 1 exon (s) 68183 - 68428 246 bp, chain + ATGGAACAAAGCTGGGCAGAGAATGACTTTGACGAGTTGAGAGAGGAAGGCTTCAGAAGA TCAAACTACTCCAAGCTAAAGGAGGAAGTTCGAACAAACGGCAAAGAAGTAAAAAACTTT GAAAAAAAATTAGATGAATGGATAACTAGAATAACCAATGCACAGAAGTCCTTAAAGGAC CTGATGGAGCTGAAAACCAAGGCAGGAGAACTACGTGACAAATACACAAGCCTCAGTAAC CGATGA >FGENESH:[exon] Gene: 7 Exon: 1 Pos: 68183 - 68428 246 bp., chain + ATGGAACAAAGCTGGGCAGAGAATGACTTTGACGAGTTGAGAGAGGAAGGCTTCAGAAGA TCAAACTACTCCAAGCTAAAGGAGGAAGTTCGAACAAACGGCAAAGAAGTAAAAAACTTT GAAAAAAAATTAGATGAATGGATAACTAGAATAACCAATGCACAGAAGTCCTTAAAGGAC CTGATGGAGCTGAAAACCAAGGCAGGAGAACTACGTGACAAATACACAAGCCTCAGTAAC CGATGA >FGENESH: 7 1 exon (s) 68183 - 68428 81 aa, chain + MEQSWAENDFDELREEGFRRSNYSKLKEEVRTNGKEVKNFEKKLDEWITRITNAQKSLKD LMELKTKAGELRDKYTSLSNR >FGENESH:[mRNA] 8 1 exon (s) 69467 - 70072 606 bp, chain + ATGGCAAAGGGATCTATTCAAGAAGAAGAACTAACTATACTAAATATATATGCACCCAAT ACAGGAGCACCCAGATTCATAAAACAAGTCCTGAGTGACCTACAAAGAGACTTAGATGCC CACACAATAATAATGGGAGACTTTAACACCCCACTGTCAACATTAGACAGATCAACGAGA CAGAAAGTTAACAAGGATATCCAGGAATTGGACTCAGCTCTGCACCAAGCAGACCTAATA GACATCTACAGAACTCTCCACCCCAAATCAACAGAATATACATTCTTTTCAGCACCACAC CACACCTATTCCAAAACTGACCACATAGTTGGAAGTAAAGCTCTCCTCAGCAAATGTAAA AGAACAGAAACTATAACAAACTGTCTCTCAGACCACAGTGCAATCAAACTAGAACTCAGG ATTAAGAAACTCACTCAAAACCACTCAGCTACATGGAAACTGAACAGCCTGCTCCTGAAT GACTACTGGGTACATAACAAAATGAAGGCAGAAATAAAGATGTTCTTTGAAACAACGAGA ACAAAGACACAACACACCAGAATCTCTGAGACACATTCAAAGCAGTGTGTAGAGGGAAAT TTATAG >FGENESH:[exon] Gene: 8 Exon: 1 Pos: 69467 - 70072 606 bp., chain + ATGGCAAAGGGATCTATTCAAGAAGAAGAACTAACTATACTAAATATATATGCACCCAAT ACAGGAGCACCCAGATTCATAAAACAAGTCCTGAGTGACCTACAAAGAGACTTAGATGCC CACACAATAATAATGGGAGACTTTAACACCCCACTGTCAACATTAGACAGATCAACGAGA CAGAAAGTTAACAAGGATATCCAGGAATTGGACTCAGCTCTGCACCAAGCAGACCTAATA GACATCTACAGAACTCTCCACCCCAAATCAACAGAATATACATTCTTTTCAGCACCACAC CACACCTATTCCAAAACTGACCACATAGTTGGAAGTAAAGCTCTCCTCAGCAAATGTAAA AGAACAGAAACTATAACAAACTGTCTCTCAGACCACAGTGCAATCAAACTAGAACTCAGG ATTAAGAAACTCACTCAAAACCACTCAGCTACATGGAAACTGAACAGCCTGCTCCTGAAT GACTACTGGGTACATAACAAAATGAAGGCAGAAATAAAGATGTTCTTTGAAACAACGAGA ACAAAGACACAACACACCAGAATCTCTGAGACACATTCAAAGCAGTGTGTAGAGGGAAAT TTATAG >FGENESH: 8 1 exon (s) 69467 - 70072 201 aa, chain + MAKGSIQEEELTILNIYAPNTGAPRFIKQVLSDLQRDLDAHTIIMGDFNTPLSTLDRSTR QKVNKDIQELDSALHQADLIDIYRTLHPKSTEYTFFSAPHHTYSKTDHIVGSKALLSKCK RTETITNCLSDHSAIKLELRIKKLTQNHSATWKLNSLLLNDYWVHNKMKAEIKMFFETTR TKTQHTRISETHSKQCVEGNL >FGENESH:[mRNA] 9 1 exon (s) 70355 - 70819 465 bp, chain + ATGACACGGGGTATCACCACTGATCCCACAGAAATACAAACTACCGTCAGAGAATACTAT AAACACCTCTACGCAAATAAACTAGAAAATCTAGAAGAAATGGATAAATTCCTCGACACA TACACTCTGCCAAGACTAAACCAGGAAGAAGTTGTATCTCTGAATAGACCAATAACAGGC TCTGAAATTGAGGCAATAATTAATAGCTTATCAACCAAAAAAAGTCCGGGACCAGTAGGA TTCATAGCCGAATTCTACCAGAGGTACAAGGAGGAGCTGGTACCATTCCTTCTGAAACTA TTCCAATCAATAGAAAAAGAGGGAATCCTCCCTAACTCATTTTATGAGGCCAGCATCATC CTGATACCAAAGCCTGACAGAGACACAACAAAAAAAGAGAATGTTACACCAATATCCTTG ATGAACATCGATGCAAAAATCCTCAATAAAATACTGGCAAACTGA >FGENESH:[exon] Gene: 9 Exon: 1 Pos: 70355 - 70819 465 bp., chain + ATGACACGGGGTATCACCACTGATCCCACAGAAATACAAACTACCGTCAGAGAATACTAT AAACACCTCTACGCAAATAAACTAGAAAATCTAGAAGAAATGGATAAATTCCTCGACACA TACACTCTGCCAAGACTAAACCAGGAAGAAGTTGTATCTCTGAATAGACCAATAACAGGC TCTGAAATTGAGGCAATAATTAATAGCTTATCAACCAAAAAAAGTCCGGGACCAGTAGGA TTCATAGCCGAATTCTACCAGAGGTACAAGGAGGAGCTGGTACCATTCCTTCTGAAACTA TTCCAATCAATAGAAAAAGAGGGAATCCTCCCTAACTCATTTTATGAGGCCAGCATCATC CTGATACCAAAGCCTGACAGAGACACAACAAAAAAAGAGAATGTTACACCAATATCCTTG ATGAACATCGATGCAAAAATCCTCAATAAAATACTGGCAAACTGA >FGENESH: 9 1 exon (s) 70355 - 70819 154 aa, chain + MTRGITTDPTEIQTTVREYYKHLYANKLENLEEMDKFLDTYTLPRLNQEEVVSLNRPITG SEIEAIINSLSTKKSPGPVGFIAEFYQRYKEELVPFLLKLFQSIEKEGILPNSFYEASII LIPKPDRDTTKKENVTPISLMNIDAKILNKILAN >FGENESH:[mRNA] 10 1 exon (s) 72135 - 72395 261 bp, chain + ATGGGCAAGGACTTCATGTCTAAAACACCAAAACGAATGGCAACAAAAGACAAAATGGAC AAACGGGATCTAATTAAACTAAAGAGCTTCTGCACAGCTAAAGAAACTACCATCAGAGTG AACAGGCAACCTACAAAATGGGAGAAAATTTTTGCAATCTACTCATCTGACAAAGGGCTA ATATCCAGAATCTACAATGAACTCAAACAAATTTACAAGAAAAAACAAACAACCCCATCA AAAAGTGGGCAAAGGATATGA >FGENESH:[exon] Gene: 10 Exon: 1 Pos: 72135 - 72395 261 bp., chain + ATGGGCAAGGACTTCATGTCTAAAACACCAAAACGAATGGCAACAAAAGACAAAATGGAC AAACGGGATCTAATTAAACTAAAGAGCTTCTGCACAGCTAAAGAAACTACCATCAGAGTG AACAGGCAACCTACAAAATGGGAGAAAATTTTTGCAATCTACTCATCTGACAAAGGGCTA ATATCCAGAATCTACAATGAACTCAAACAAATTTACAAGAAAAAACAAACAACCCCATCA AAAAGTGGGCAAAGGATATGA >FGENESH: 10 1 exon (s) 72135 - 72395 86 aa, chain + MGKDFMSKTPKRMATKDKMDKRDLIKLKSFCTAKETTIRVNRQPTKWEKIFAIYSSDKGL ISRIYNELKQIYKKKQTTPSKSGQRI
G - predicted gene number, starting from start of sequence;
Str - DNA strand (+ for direct or - for complementary);
Feature - Type (feature of coding sequence):
CDSf - first (starting with start codon),
CDSi - internal (internal exon), CDSl - last (ending with stop codon) coding segment,
CDSo - gene contains the ONE coding exon only;
Start and End - Position of the Feature;
Score - Log likelihood*10 score for the feature;
ORF - start/end positions where the first codon starts and the last codon ends.
Len - length of the coding segment.
PolA - poly(A) site