Fgenesh

Program for predicting multiple genes in genomic DNA sequences.

Fgenesh is the fastest (50-100 times faster than GenScan) and most accurate gene finder available (see: Figure and Table, respectively). In recent rice genome sequencing projects, it was cited "the most successful (gene finding) program (Yu et al. (2002) Science 296:79) and was used to produce 87% of all high-evidence predicted genes (Goff et al. (2002) Science 296:79).

 

   

Figure. Performance of different gene finding programs on rice genes (reprinted from Yu et al., 2002, Science, 296:79-92). These tests confirmed that Fgenesh is by far the most accurate program (of five programs tested).

Table. Performance of three popular gene prediction programs on 42 semi-artificial genomic sequences containing 178 known human gene sequences (900 exons). Sensitivity is percentage of exons that are predicted correctly. Selectivity is percentage of predicted exons that are correct (these results reproduced with some changes from Yada et al., 2002, Cold Spring Harbor Genome Sequencing and Biology Meeting, May 7-11). These tests demonstrated that Fgenesh is by far the most accurate program (of three programs tested).

Program
Sensitivity
Specificity
Missed Exons, %
Wrong Exons, %
Fgenesh
77.1
65.7
9.6
23.2
GenScan
66.5
44.9
12.0
40.9
HMMGene
69.6
36.6
15.5
55.5

 

References: Salamov A., Solovyev V. (2000)
Ab initio gene finding in Drosophila genomic DNA.
Genome Res., 10,516-522

Fgenesh output:


 FGENESH 2.6 Prediction of potential genes in Homo_sapiens genomic DNA
 Time    :   Thu Dec 27 19:47:24 2007
 Seq name: gi|13907843|ref|NG_000007.1| Homo sapiens genomic beta globin region (HBB@) on chromosome 11  
 Length of sequence: 73308 
 Number of predicted genes 10: in +chain 10, in -chain 0.
 Number of predicted exons 21: in +chain 21, in -chain 0.
 Positions of predicted genes and exons: Variant   1 from   1, Score:180.171899 
   G Str   Feature   Start        End    Score           ORF           Len

   1 +      TSS      19456               -7.09
   1 +    1 CDSf     19541 -     19632   16.13     19541 -     19630     90
   1 +    2 CDSi     19755 -     19977   13.37     19756 -     19977    222
   1 +    3 CDSl     20833 -     20961    3.34     20833 -     20961    129
   1 +      PolA     21055                1.13

   2 +      TSS      34446               -7.09
   2 +    1 CDSf     34531 -     34622   13.42     34531 -     34620     90
   2 +    2 CDSi     34745 -     34967   21.52     34746 -     34967    222
   2 +    3 CDSl     35854 -     35982    2.92     35854 -     35982    129
   2 +      PolA     36043                1.13

   3 +      TSS      39382               -7.09
   3 +    1 CDSf     39467 -     39558   13.42     39467 -     39556     90
   3 +    2 CDSi     39681 -     39903   21.52     39682 -     39903    222
   3 +    3 CDSl     40770 -     40898    3.66     40770 -     40898    129
   3 +      PolA     40959                1.13

   4 +      TSS      44415               -8.69
   4 +    1 CDSf     45995 -     46151   16.58     45995 -     46150    156
   4 +    2 CDSl     46997 -     47100   -1.94     46999 -     47100    102
   4 +      PolA     47243                1.13

   5 +      TSS      54707               -4.39
   5 +    1 CDSf     54790 -     54881   13.44     54790 -     54879     90
   5 +    2 CDSi     55010 -     55232   17.01     55011 -     55232    222
   5 +    3 CDSl     56425 -     56535    2.53     56425 -     56535    111
   5 +      PolA     56931                1.13

   6 +      TSS      62104               -6.59
   6 +    1 CDSf     62187 -     62278   12.99     62187 -     62276     90
   6 +    2 CDSi     62409 -     62631   20.06     62410 -     62631    222
   6 +    3 CDSl     63482 -     63610    9.54     63482 -     63610    129
   6 +      PolA     63718                1.13

   7 +      TSS      68088               -9.39
   7 +    1 CDSo     68183 -     68428   19.52     68183 -     68428    246
   7 +      PolA     68509                1.13

   8 +      TSS      69336              -10.29
   8 +    1 CDSo     69467 -     70072   16.45     69467 -     70072    606
   8 +      PolA     70131               -1.08

   9 +      TSS      70224              -12.49
   9 +    1 CDSo     70355 -     70819   17.10     70355 -     70819    465
   9 +      PolA     70905                1.13

  10 +      TSS      72085               -6.39
  10 +    1 CDSo     72135 -     72395    7.31     72135 -     72395    261
  10 +      PolA     72952                1.13

Predicted protein(s):
>FGENESH:[mRNA]   1   3 exon (s)  19541  -  20961   444 bp, chain +
ATGGTGCATTTTACTGCTGAGGAGAAGGCTGCCGTCACTAGCCTGTGGAGCAAGATGAAT
GTGGAAGAGGCTGGAGGTGAAGCCTTGGGCAGACTCCTCGTTGTTTACCCCTGGACCCAG
AGATTTTTTGACAGCTTTGGAAACCTGTCGTCTCCCTCTGCCATCCTGGGCAACCCCAAG
GTCAAGGCCCATGGCAAGAAGGTGCTGACTTCCTTTGGAGATGCTATTAAAAACATGGAC
AACCTCAAGCCCGCCTTTGCTAAGCTGAGTGAGCTGCACTGTGACAAGCTGCATGTGGAT
CCTGAGAACTTCAAGCTCCTGGGTAACGTGATGGTGATTATTCTGGCTACTCACTTTGGC
AAGGAGTTCACCCCTGAAGTGCAGGCTGCCTGGCAGAAGCTGGTGTCTGCTGTCGCCATT
GCCCTGGCCCATAAGTACCACTGA
>FGENESH:[exon] Gene:  1 Exon:  1  Pos: 19541  -  19632    92 bp., chain +
ATGGTGCATTTTACTGCTGAGGAGAAGGCTGCCGTCACTAGCCTGTGGAGCAAGATGAAT
GTGGAAGAGGCTGGAGGTGAAGCCTTGGGCAG
>FGENESH:[exon] Gene:  1 Exon:  2  Pos: 19755  -  19977   223 bp., chain +
ACTCCTCGTTGTTTACCCCTGGACCCAGAGATTTTTTGACAGCTTTGGAAACCTGTCGTC
TCCCTCTGCCATCCTGGGCAACCCCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGACTTC
CTTTGGAGATGCTATTAAAAACATGGACAACCTCAAGCCCGCCTTTGCTAAGCTGAGTGA
GCTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAG
>FGENESH:[exon] Gene:  1 Exon:  3  Pos: 20833  -  20961   129 bp., chain +
CTCCTGGGTAACGTGATGGTGATTATTCTGGCTACTCACTTTGGCAAGGAGTTCACCCCT
GAAGTGCAGGCTGCCTGGCAGAAGCTGGTGTCTGCTGTCGCCATTGCCCTGGCCCATAAG
TACCACTGA
>FGENESH:   1   3 exon (s)  19541  -  20961   147 aa, chain +
MVHFTAEEKAAVTSLWSKMNVEEAGGEALGRLLVVYPWTQRFFDSFGNLSSPSAILGNPK
VKAHGKKVLTSFGDAIKNMDNLKPAFAKLSELHCDKLHVDPENFKLLGNVMVIILATHFG
KEFTPEVQAAWQKLVSAVAIALAHKYH
>FGENESH:[mRNA]   2   3 exon (s)  34531  -  35982   444 bp, chain +
ATGGGTCATTTCACAGAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAAT
GTGGAAGATGCTGGAGGAGAAACCCTGGGAAGGCTCCTGGTTGTCTACCCATGGACCCAG
AGGTTCTTTGACAGCTTTGGCAACCTGTCCTCTGCCTCTGCCATCATGGGCAACCCCAAA
GTCAAGGCACATGGCAAGAAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGAT
GATCTCAAGGGCACCTTTGCCCAGCTGAGTGAACTGCACTGTGACAAGCTGCATGTGGAT
CCTGAGAACTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCGGC
AAAGAATTCACCCCTGAGGTGCAGGCTTCCTGGCAGAAGATGGTGACTGGAGTGGCCAGT
GCCCTGTCCTCCAGATACCACTGA
>FGENESH:[exon] Gene:  2 Exon:  1  Pos: 34531  -  34622    92 bp., chain +
ATGGGTCATTTCACAGAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAAT
GTGGAAGATGCTGGAGGAGAAACCCTGGGAAG
>FGENESH:[exon] Gene:  2 Exon:  2  Pos: 34745  -  34967   223 bp., chain +
GCTCCTGGTTGTCTACCCATGGACCCAGAGGTTCTTTGACAGCTTTGGCAACCTGTCCTC
TGCCTCTGCCATCATGGGCAACCCCAAAGTCAAGGCACATGGCAAGAAGGTGCTGACTTC
CTTGGGAGATGCCATAAAGCACCTGGATGATCTCAAGGGCACCTTTGCCCAGCTGAGTGA
ACTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAG
>FGENESH:[exon] Gene:  2 Exon:  3  Pos: 35854  -  35982   129 bp., chain +
CTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCGGCAAAGAATTCACCCCT
GAGGTGCAGGCTTCCTGGCAGAAGATGGTGACTGGAGTGGCCAGTGCCCTGTCCTCCAGA
TACCACTGA
>FGENESH:   2   3 exon (s)  34531  -  35982   147 aa, chain +
MGHFTEEDKATITSLWGKVNVEDAGGETLGRLLVVYPWTQRFFDSFGNLSSASAIMGNPK
VKAHGKKVLTSLGDAIKHLDDLKGTFAQLSELHCDKLHVDPENFKLLGNVLVTVLAIHFG
KEFTPEVQASWQKMVTGVASALSSRYH
>FGENESH:[mRNA]   3   3 exon (s)  39467  -  40898   444 bp, chain +
ATGGGTCATTTCACAGAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAAT
GTGGAAGATGCTGGAGGAGAAACCCTGGGAAGGCTCCTGGTTGTCTACCCATGGACCCAG
AGGTTCTTTGACAGCTTTGGCAACCTGTCCTCTGCCTCTGCCATCATGGGCAACCCCAAA
GTCAAGGCACATGGCAAGAAGGTGCTGACTTCCTTGGGAGATGCCATAAAGCACCTGGAT
GATCTCAAGGGCACCTTTGCCCAGCTGAGTGAACTGCACTGTGACAAGCTGCATGTGGAT
CCTGAGAACTTCAAGCTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCGGC
AAAGAATTCACCCCTGAGGTGCAGGCTTCCTGGCAGAAGATGGTGACTGCAGTGGCCAGT
GCCCTGTCCTCCAGATACCACTGA
>FGENESH:[exon] Gene:  3 Exon:  1  Pos: 39467  -  39558    92 bp., chain +
ATGGGTCATTTCACAGAGGAGGACAAGGCTACTATCACAAGCCTGTGGGGCAAGGTGAAT
GTGGAAGATGCTGGAGGAGAAACCCTGGGAAG
>FGENESH:[exon] Gene:  3 Exon:  2  Pos: 39681  -  39903   223 bp., chain +
GCTCCTGGTTGTCTACCCATGGACCCAGAGGTTCTTTGACAGCTTTGGCAACCTGTCCTC
TGCCTCTGCCATCATGGGCAACCCCAAAGTCAAGGCACATGGCAAGAAGGTGCTGACTTC
CTTGGGAGATGCCATAAAGCACCTGGATGATCTCAAGGGCACCTTTGCCCAGCTGAGTGA
ACTGCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAAG
>FGENESH:[exon] Gene:  3 Exon:  3  Pos: 40770  -  40898   129 bp., chain +
CTCCTGGGAAATGTGCTGGTGACCGTTTTGGCAATCCATTTCGGCAAAGAATTCACCCCT
GAGGTGCAGGCTTCCTGGCAGAAGATGGTGACTGCAGTGGCCAGTGCCCTGTCCTCCAGA
TACCACTGA
>FGENESH:   3   3 exon (s)  39467  -  40898   147 aa, chain +
MGHFTEEDKATITSLWGKVNVEDAGGETLGRLLVVYPWTQRFFDSFGNLSSASAIMGNPK
VKAHGKKVLTSLGDAIKHLDDLKGTFAQLSELHCDKLHVDPENFKLLGNVLVTVLAIHFG
KEFTPEVQASWQKMVTAVASALSSRYH
>FGENESH:[mRNA]   4   2 exon (s)  45995  -  47100   261 bp, chain +
ATGGGCAACCCCAAAGTCAAGGCACATGGCAAGAAGGTGCTGATCTCCTTCGGAAAAGCT
GTTATGCTCACGGATGACCTCAAAGGCACCTTTGCTACACTGAGTGACCTGCACTGTAAC
AAGCTGCACGTGGACCCTGAGAACTTCCTGGTGAGTACTCTTAGGCAACGTGATATTGAT
TGTTTTGGCAACCCACTTCAGCGAGGATTTTACCCTACAGATACAGGCTTCTTGGCAGTA
ACTAACAAATGCTGTGGTTAA
>FGENESH:[exon] Gene:  4 Exon:  1  Pos: 45995  -  46151   157 bp., chain +
ATGGGCAACCCCAAAGTCAAGGCACATGGCAAGAAGGTGCTGATCTCCTTCGGAAAAGCT
GTTATGCTCACGGATGACCTCAAAGGCACCTTTGCTACACTGAGTGACCTGCACTGTAAC
AAGCTGCACGTGGACCCTGAGAACTTCCTGGTGAGTA
>FGENESH:[exon] Gene:  4 Exon:  2  Pos: 46997  -  47100   104 bp., chain +
CTCTTAGGCAACGTGATATTGATTGTTTTGGCAACCCACTTCAGCGAGGATTTTACCCTA
CAGATACAGGCTTCTTGGCAGTAACTAACAAATGCTGTGGTTAA
>FGENESH:   4   2 exon (s)  45995  -  47100    86 aa, chain +
MGNPKVKAHGKKVLISFGKAVMLTDDLKGTFATLSDLHCNKLHVDPENFLVSTLRQRDID
CFGNPLQRGFYPTDTGFLAVTNKCCG
>FGENESH:[mRNA]   5   3 exon (s)  54790  -  56535   426 bp, chain +
ATGGTGCATCTGACTCCTGAGGAGAAGACTGCTGTCAATGCCCTGTGGGGCAAAGTGAAC
GTGGATGCAGTTGGTGGTGAGGCCCTGGGCAGATTACTGGTGGTCTACCCTTGGACCCAG
AGGTTCTTTGAGTCCTTTGGGGATCTGTCCTCTCCTGATGCTGTTATGGGCAACCCTAAG
GTGAAGGCTCATGGCAAGAAGGTGCTAGGTGCCTTTAGTGATGGCCTGGCTCACCTGGAC
AACCTCAAGGGCACTTTTTCTCAGCTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGAT
CCTGAGAACTTCAGGGTGTGTAAGAAGGTTCCTGAGGCTCTACAGATAGGGAGCACTTGT
TTATTTTACAAAGAGTACATGGGAAAAGAGAAAAGCAAGGGAACCGTACAAGGCATTAAT
GGGTGA
>FGENESH:[exon] Gene:  5 Exon:  1  Pos: 54790  -  54881    92 bp., chain +
ATGGTGCATCTGACTCCTGAGGAGAAGACTGCTGTCAATGCCCTGTGGGGCAAAGTGAAC
GTGGATGCAGTTGGTGGTGAGGCCCTGGGCAG
>FGENESH:[exon] Gene:  5 Exon:  2  Pos: 55010  -  55232   223 bp., chain +
ATTACTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCTC
TCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAGGTGCTAGGTGC
CTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACTTTTTCTCAGCTGAGTGA
GCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGG
>FGENESH:[exon] Gene:  5 Exon:  3  Pos: 56425  -  56535   111 bp., chain +
GTGTGTAAGAAGGTTCCTGAGGCTCTACAGATAGGGAGCACTTGTTTATTTTACAAAGAG
TACATGGGAAAAGAGAAAAGCAAGGGAACCGTACAAGGCATTAATGGGTGA
>FGENESH:   5   3 exon (s)  54790  -  56535   141 aa, chain +
MVHLTPEEKTAVNALWGKVNVDAVGGEALGRLLVVYPWTQRFFESFGDLSSPDAVMGNPK
VKAHGKKVLGAFSDGLAHLDNLKGTFSQLSELHCDKLHVDPENFRVCKKVPEALQIGSTC
LFYKEYMGKEKSKGTVQGING
>FGENESH:[mRNA]   6   3 exon (s)  62187  -  63610   444 bp, chain +
ATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAAC
GTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTACCCTTGGACCCAG
AGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATGCTGTTATGGGCAACCCTAAG
GTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGAC
AACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGAT
CCTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGC
AAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAAT
GCCCTGGCCCACAAGTATCACTAA
>FGENESH:[exon] Gene:  6 Exon:  1  Pos: 62187  -  62278    92 bp., chain +
ATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAAC
GTGGATGAAGTTGGTGGTGAGGCCCTGGGCAG
>FGENESH:[exon] Gene:  6 Exon:  2  Pos: 62409  -  62631   223 bp., chain +
GCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCAC
TCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC
CTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGA
GCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGG
>FGENESH:[exon] Gene:  6 Exon:  3  Pos: 63482  -  63610   129 bp., chain +
CTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCA
CCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAG
TATCACTAA
>FGENESH:   6   3 exon (s)  62187  -  63610   147 aa, chain +
MVHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPK
VKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFG
KEFTPPVQAAYQKVVAGVANALAHKYH
>FGENESH:[mRNA]   7   1 exon (s)  68183  -  68428   246 bp, chain +
ATGGAACAAAGCTGGGCAGAGAATGACTTTGACGAGTTGAGAGAGGAAGGCTTCAGAAGA
TCAAACTACTCCAAGCTAAAGGAGGAAGTTCGAACAAACGGCAAAGAAGTAAAAAACTTT
GAAAAAAAATTAGATGAATGGATAACTAGAATAACCAATGCACAGAAGTCCTTAAAGGAC
CTGATGGAGCTGAAAACCAAGGCAGGAGAACTACGTGACAAATACACAAGCCTCAGTAAC
CGATGA
>FGENESH:[exon] Gene:  7 Exon:  1  Pos: 68183  -  68428   246 bp., chain +
ATGGAACAAAGCTGGGCAGAGAATGACTTTGACGAGTTGAGAGAGGAAGGCTTCAGAAGA
TCAAACTACTCCAAGCTAAAGGAGGAAGTTCGAACAAACGGCAAAGAAGTAAAAAACTTT
GAAAAAAAATTAGATGAATGGATAACTAGAATAACCAATGCACAGAAGTCCTTAAAGGAC
CTGATGGAGCTGAAAACCAAGGCAGGAGAACTACGTGACAAATACACAAGCCTCAGTAAC
CGATGA
>FGENESH:   7   1 exon (s)  68183  -  68428    81 aa, chain +
MEQSWAENDFDELREEGFRRSNYSKLKEEVRTNGKEVKNFEKKLDEWITRITNAQKSLKD
LMELKTKAGELRDKYTSLSNR
>FGENESH:[mRNA]   8   1 exon (s)  69467  -  70072   606 bp, chain +
ATGGCAAAGGGATCTATTCAAGAAGAAGAACTAACTATACTAAATATATATGCACCCAAT
ACAGGAGCACCCAGATTCATAAAACAAGTCCTGAGTGACCTACAAAGAGACTTAGATGCC
CACACAATAATAATGGGAGACTTTAACACCCCACTGTCAACATTAGACAGATCAACGAGA
CAGAAAGTTAACAAGGATATCCAGGAATTGGACTCAGCTCTGCACCAAGCAGACCTAATA
GACATCTACAGAACTCTCCACCCCAAATCAACAGAATATACATTCTTTTCAGCACCACAC
CACACCTATTCCAAAACTGACCACATAGTTGGAAGTAAAGCTCTCCTCAGCAAATGTAAA
AGAACAGAAACTATAACAAACTGTCTCTCAGACCACAGTGCAATCAAACTAGAACTCAGG
ATTAAGAAACTCACTCAAAACCACTCAGCTACATGGAAACTGAACAGCCTGCTCCTGAAT
GACTACTGGGTACATAACAAAATGAAGGCAGAAATAAAGATGTTCTTTGAAACAACGAGA
ACAAAGACACAACACACCAGAATCTCTGAGACACATTCAAAGCAGTGTGTAGAGGGAAAT
TTATAG
>FGENESH:[exon] Gene:  8 Exon:  1  Pos: 69467  -  70072   606 bp., chain +
ATGGCAAAGGGATCTATTCAAGAAGAAGAACTAACTATACTAAATATATATGCACCCAAT
ACAGGAGCACCCAGATTCATAAAACAAGTCCTGAGTGACCTACAAAGAGACTTAGATGCC
CACACAATAATAATGGGAGACTTTAACACCCCACTGTCAACATTAGACAGATCAACGAGA
CAGAAAGTTAACAAGGATATCCAGGAATTGGACTCAGCTCTGCACCAAGCAGACCTAATA
GACATCTACAGAACTCTCCACCCCAAATCAACAGAATATACATTCTTTTCAGCACCACAC
CACACCTATTCCAAAACTGACCACATAGTTGGAAGTAAAGCTCTCCTCAGCAAATGTAAA
AGAACAGAAACTATAACAAACTGTCTCTCAGACCACAGTGCAATCAAACTAGAACTCAGG
ATTAAGAAACTCACTCAAAACCACTCAGCTACATGGAAACTGAACAGCCTGCTCCTGAAT
GACTACTGGGTACATAACAAAATGAAGGCAGAAATAAAGATGTTCTTTGAAACAACGAGA
ACAAAGACACAACACACCAGAATCTCTGAGACACATTCAAAGCAGTGTGTAGAGGGAAAT
TTATAG
>FGENESH:   8   1 exon (s)  69467  -  70072   201 aa, chain +
MAKGSIQEEELTILNIYAPNTGAPRFIKQVLSDLQRDLDAHTIIMGDFNTPLSTLDRSTR
QKVNKDIQELDSALHQADLIDIYRTLHPKSTEYTFFSAPHHTYSKTDHIVGSKALLSKCK
RTETITNCLSDHSAIKLELRIKKLTQNHSATWKLNSLLLNDYWVHNKMKAEIKMFFETTR
TKTQHTRISETHSKQCVEGNL
>FGENESH:[mRNA]   9   1 exon (s)  70355  -  70819   465 bp, chain +
ATGACACGGGGTATCACCACTGATCCCACAGAAATACAAACTACCGTCAGAGAATACTAT
AAACACCTCTACGCAAATAAACTAGAAAATCTAGAAGAAATGGATAAATTCCTCGACACA
TACACTCTGCCAAGACTAAACCAGGAAGAAGTTGTATCTCTGAATAGACCAATAACAGGC
TCTGAAATTGAGGCAATAATTAATAGCTTATCAACCAAAAAAAGTCCGGGACCAGTAGGA
TTCATAGCCGAATTCTACCAGAGGTACAAGGAGGAGCTGGTACCATTCCTTCTGAAACTA
TTCCAATCAATAGAAAAAGAGGGAATCCTCCCTAACTCATTTTATGAGGCCAGCATCATC
CTGATACCAAAGCCTGACAGAGACACAACAAAAAAAGAGAATGTTACACCAATATCCTTG
ATGAACATCGATGCAAAAATCCTCAATAAAATACTGGCAAACTGA
>FGENESH:[exon] Gene:  9 Exon:  1  Pos: 70355  -  70819   465 bp., chain +
ATGACACGGGGTATCACCACTGATCCCACAGAAATACAAACTACCGTCAGAGAATACTAT
AAACACCTCTACGCAAATAAACTAGAAAATCTAGAAGAAATGGATAAATTCCTCGACACA
TACACTCTGCCAAGACTAAACCAGGAAGAAGTTGTATCTCTGAATAGACCAATAACAGGC
TCTGAAATTGAGGCAATAATTAATAGCTTATCAACCAAAAAAAGTCCGGGACCAGTAGGA
TTCATAGCCGAATTCTACCAGAGGTACAAGGAGGAGCTGGTACCATTCCTTCTGAAACTA
TTCCAATCAATAGAAAAAGAGGGAATCCTCCCTAACTCATTTTATGAGGCCAGCATCATC
CTGATACCAAAGCCTGACAGAGACACAACAAAAAAAGAGAATGTTACACCAATATCCTTG
ATGAACATCGATGCAAAAATCCTCAATAAAATACTGGCAAACTGA
>FGENESH:   9   1 exon (s)  70355  -  70819   154 aa, chain +
MTRGITTDPTEIQTTVREYYKHLYANKLENLEEMDKFLDTYTLPRLNQEEVVSLNRPITG
SEIEAIINSLSTKKSPGPVGFIAEFYQRYKEELVPFLLKLFQSIEKEGILPNSFYEASII
LIPKPDRDTTKKENVTPISLMNIDAKILNKILAN
>FGENESH:[mRNA]  10   1 exon (s)  72135  -  72395   261 bp, chain +
ATGGGCAAGGACTTCATGTCTAAAACACCAAAACGAATGGCAACAAAAGACAAAATGGAC
AAACGGGATCTAATTAAACTAAAGAGCTTCTGCACAGCTAAAGAAACTACCATCAGAGTG
AACAGGCAACCTACAAAATGGGAGAAAATTTTTGCAATCTACTCATCTGACAAAGGGCTA
ATATCCAGAATCTACAATGAACTCAAACAAATTTACAAGAAAAAACAAACAACCCCATCA
AAAAGTGGGCAAAGGATATGA
>FGENESH:[exon] Gene: 10 Exon:  1  Pos: 72135  -  72395   261 bp., chain +
ATGGGCAAGGACTTCATGTCTAAAACACCAAAACGAATGGCAACAAAAGACAAAATGGAC
AAACGGGATCTAATTAAACTAAAGAGCTTCTGCACAGCTAAAGAAACTACCATCAGAGTG
AACAGGCAACCTACAAAATGGGAGAAAATTTTTGCAATCTACTCATCTGACAAAGGGCTA
ATATCCAGAATCTACAATGAACTCAAACAAATTTACAAGAAAAAACAAACAACCCCATCA
AAAAGTGGGCAAAGGATATGA
>FGENESH:  10   1 exon (s)  72135  -  72395    86 aa, chain +
MGKDFMSKTPKRMATKDKMDKRDLIKLKSFCTAKETTIRVNRQPTKWEKIFAIYSSDKGL
ISRIYNELKQIYKKKQTTPSKSGQRI

Where:

G - predicted gene number, starting from start of sequence;
Str - DNA strand (+ for direct or - for complementary);
Feature - Type (feature of coding sequence): CDSf - first (starting with start codon), CDSi - internal (internal exon), CDSl - last (ending with stop codon) coding segment, CDSo - gene contains the ONE coding exon only;
Start and End - Position of the Feature;
Score - Log likelihood*10 score for the feature;
ORF - start/end positions where the first codon starts and the last codon ends.
Len - length of the coding segment.
PolA - poly(A) site