Blast2seq

Input
Query sequence First input file
Target sequence Second input file
Output
Result   Designates an output file for the search results.
Options
Program name Select search program.
  • Blastp - compares an amino acid query sequence against a protein sequence database.
  • Blastn - compares a nucleotide query sequence against a nucleotide sequence database.
  • Blastx - compares the six-frame conceptual translation products of a nucleotide query sequence (both strands) against a protein sequence database.
  • tBlastn - compares a protein query sequence against a nucleotide sequence database dynamically translated in all six reading frames (both strands).
  • tBlastx - compares the six-frame translations of a nucleotide query sequence against the six-frame translations of a nucleotide sequence database.
    For blastx 1st sequence should be nucleotide, tblastn 2nd sequence nucleotide.
  • Expectation value   Sets the threshold expectation value for keeping alignments.
    This is the E from the Karlin-Altschul equation that describes how often an alignment with a given score is expected to occur at random.